Competitive RNA Templates for Detection and Quantitation of Growth Factors, Cytokines, Extracellular Matrix Components and Matrix Metalloproteinases by RT-PCR

Competitive RNA Templates for Detection and Quantitation of Growth Factors, Cytokines, Extracellular Matrix Components and Matrix Metalloproteinases by RT-PCR

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Detection of low-abundance mRNAs by reverse transcription-polymerase chain reaction (RT-PCR) has become a standard technique to determine gene expression by tissues and cells in culture.The ability to determine relative or absolute copy number of specific mRNAs has been difJicult due to Hockey Protective - Gloves - Youth inadequate internal standards to control for sample-to-sample variation.The use of a synthetic RNA standard with identical sequences to the PCR primers allows reproducible quantitation between samples and assays.By designing multi-sequence templates, several specijic mRNAs can be quantitated using a single template.Addition of multiple templates to a single RT reaction allows the quantitation of a large number of targetsffom as little as 4 pg of total RNA.

In this report, we present Electronic Circuit Board a series of seven primer/template systems to detect and quantitate 52 specific messages, including 26 growth factors and receptors, 8 extracellular matrix components, 10 matrix-rnodifying enzymes and their inhibitors and 8 cytokines.