Competitive RNA Templates for Detection and Quantitation of Growth Factors, Cytokines, Extracellular Matrix Components and Matrix Metalloproteinases by RT-PCR
Detection of low-abundance mRNAs by reverse transcription-polymerase chain reaction (RT-PCR) has become a standard technique to determine gene expression by tissues and cells in culture.The ability to determine relative or absolute copy number of specific mRNAs has been difJicult due to Hockey Protective - Gloves - Youth inadequate internal standar